The first step is collecting the cells by a blood draw, then the peripheral blood mononuclear cells must be separated from the other blood cells by centrifugation, then the cells must be frozen and placed in liquid nitrogen for long-term storage. Image credit: Nancy R. Gough, BioSerendipity.
Read more:
N. R. Gough, A Cryobank for T Cells. BioSerendipity (19 July 2019) https://www.bioserendipity.com/a-cryobank-for-t-cells/